The course aims to provide students with a critical understanding of the various ways in which large scale protein expression can be carried out in recombinant systems for Biotechnological applications.

Lectures, tutorials and a lab class.

None

None

Lab Assessment (75%)

Group Presentation (25%) - video presentation

It is the default expectation that all courses will offer opportunities for reassessment or deferred assessment. Where it is not possible to offer this in some assessment components, the grade achieved at the first attempt will be counted towards the final course grade, and any exceptions for this course are described below.

[No exceptions]

The course aims to provide students with a critical understanding of the various ways in which large scale protein expression can be carried out for Biotechnological applications. The course will explore various recombinant systems, the use of coupled proteins and tags, expression of difficult proteins and different methods of affinity purification.

By the end of this course students will be able to:

■ Critically analyse the current methodologies used to express and purify proteins of biotechnological significance in recombinant systems.

■ Critically evaluate the use of different recombinant systems and purification mechanisms.

■ Describe, critique and perform techniques that are commonly used to express proteins in E.coli.

■ Critically analyse results from experimental data and write an accurate laboratory report using these results.